Creation of the first new artificial life form on Earth

Craig Venter, the controversial DNA researcher involved in the race to decipher the human genetic code, has built a synthetic chromosome out of laboratory chemicals and is poised to announce the creation of the first new artificial life form on Earth.

The announcement, which is expected within weeks and could come as early as Monday at the annual meeting of his scientific institute in San Diego, California, will herald a giant leap forward in the development of designer genomes. It is certain to provoke heated debate about the ethics of creating new species and could unlock the door to new energy sources and techniques to combat global warming.

Mr Venter told the Guardian he thought this landmark would be "a very important philosophical step in the history of our species. We are going from reading our genetic code to the ability to write it. That gives us the hypothetical ability to do things never contemplated before".

The Guardian can reveal that a team of 20 top scientists assembled by Mr Venter, led by the Nobel laureate Hamilton Smith, has already constructed a synthetic chromosome, a feat of virtuoso bio-engineering never previously achieved. Using lab-made chemicals, they have painstakingly stitched together a chromosome that is 381 genes long and contains 580,000 base pairs of genetic code.

The DNA sequence is based on the bacterium Mycoplasma genitalium which the team pared down to the bare essentials needed to support life, removing a fifth of its genetic make-up. The wholly synthetically reconstructed chromosome, which the team have christened Mycoplasma laboratorium, has been watermarked with inks for easy recognition.

It is then transplanted into a living bacterial cell and in the final stage of the process it is expected to take control of the cell and in effect become a new life form. The team of scientists has already successfully transplanted the genome of one type of bacterium into the cell of another, effectively changing the cell's species. Mr Venter said he was "100% confident" the same technique would work for the artificially created chromosome.

The new life form will depend for its ability to replicate itself and metabolise on the molecular machinery of the cell into which it has been injected, and in that sense it will not be a wholly synthetic life form. However, its DNA will be artificial, and it is the DNA that controls the cell and is credited with being the building block of life.

Mr Venter said he had carried out an ethical review before completing the experiment. "We feel that this is good science," he said. He has further heightened the controversy surrounding his potential breakthrough by applying for a patent for the synthetic bacterium.

Pat Mooney, director of a Canadian bioethics organisation, ETC group, said the move was an enormous challenge to society to debate the risks involved. "Governments, and society in general, is way behind the ball. This is a wake-up call, what does it mean to create new life forms in a test-tube?"

He said Mr Venter was creating a "chassis on which you could build almost anything. It could be a contribution to humanity such as new drugs or a huge threat to humanity such as bio-weapons".

Mr Venter believes designer genomes have enormous positive potential if properly regulated. In the long-term, he hopes they could lead to alternative energy sources previously unthinkable. Bacteria could be created, he speculates, that could help mop up excessive carbon dioxide, thus contributing to the solution to global warming, or produce fuels such as butane or propane made entirely from sugar.

"We are not afraid to take on things that are important just because they stimulate thinking," he said. "We are dealing in big ideas. We are trying to create a new value system for life. When dealing at this scale, you can't expect everybody to be happy."

(This article appeared in The Guardian newspaper Saturday October 6 2007).

Scientists to barcode world's species

A group of Canadian scientists is working on an ambitious project to create a global database of up to half a million of the world's species using DNA barcoding technology.

The scientists are hoping to raise $150 million to fund an initial five-year stage of what they describe as the biodiversity equivalent of launching a rocket to the moon.

DNA barcoding, a technique for characterising a species using only a short DNA sequence, has wide-ranging implications for health and environment.

It could help remove illegal fish and timber supplies from global markets, get rid of pests such as mosquitoes and even reduce the numbers of collisions between birds and planes.

Paul Hebert, head of the Canadian Centre for DNA Barcoding, is spearheading the plan. “We're now trying to launch in Canada the International Barcode of Life Project, which has a five-year life span,” Hebert said at a three-day seminar on DNA in Taipei.

“We hope to put $150 million into this through a 25 Nation Alliance.”

“The idea is collectively we would gather five million specimens and 5,00,000 species within that five-year period,” Hebert added, saying the entire project could take 15 years.

The seminar in Taipei has brought together 350 scientists from 45 countries to debate the “barcoding of life” concept.

Scientists estimate that while nearly 1.8 million species have already been identified, there may be another 10 million that are not known.

But DNA barcoding technology has progressed so rapidly that scientists predict science fiction-style powers to recognise previously unfamiliar creatures could become reality in a decade.

“Like in the film of Star Trek, anything scanned by such devices could display its image, name and function,” said Allen Chen from Taiwan's Academia Sinica.

“This could be done 10 years from now after a global barcoding data bank is set up,” said Chen, an expert in corals.

Scientists are already working on hand-held barcoders that would enable users to access a barcode data bank using a global positioning system, said Taiwan's Shao Kwang tsao, one of the conference chairs.

Hebert said the alliance would invest heavily in the development of such technology.

This week's conference is being held by the Washington based Consortium for the Barcode of Life, which was set up in 2003 in response to Hebert's initiative and now includes some 160 organisations.

Among them is Taiwan's top academic body, Academia Sinica, one of three chief organisers of the conference.

At its first conference in London in 2005, the consortium's data banks collected some 33,000 DNA references belonging to some 12,700 species.

Today it counts more than 2,90,000 DNA samples from some 31,000 species, including about 20 per cent of the world's estimated 10,000 bird species and 10 per cent of the 35,000 estimated marine and freshwater fish species.

The “barcoding of life” projects have drawn increasing attention, particularly from the US, Canada and Europe, as scientists explore the technique's applications, which range from food safety and consumer protection to the identification of herbal plants.

One British scientist is working on a project to barcode 2,800 species of mosquito or 80 per cent of those known to the world, within two years.

The project is aimed at reducing the scourge of malaria, which infects some 500 million people a year and is spread by some mosquitoes.